Bed Bam & Beyond

Among the factors responsible for scaring people away from bioinformatics, the various input and output filetypes surely rank very high. If you want to plot heat maps using ngsplot, you need a bam file. If you want to use deeptools instead, also to make heat maps, you...

Trimming for RNA-Seq data

Trimming for adaptors and low quality bases is important part of the analysis pipeline for sequencing data. Typically, after you isolate and fragment your RNA sample, adaptors are attached to the ends of the sequences that are needed for sequencing (see [1,2] for more...