Automated ChIP-Seq peak calling and alignmentGet publication-ready results within hours - not days or weeks. Basepair’s automated ChIP-Seq data analysis enables alignment, read counts (complete with trimming and deduplication numbers), peak calling, motif analysis, and interactive figures and plots to get you closer to publication. Free 14-Day Trial
What is ChIP sequencing?
ChIP-Seq refers to chromatin immunoprecipitation followed by next generation sequencing (NGS). This technique is used to find DNA-protein binding, such as transcription factor binding sites, histone modification, open chromatin regions, and more. The core idea behind each of these techniques is to cross-link and immunoprecipitate the epitope of interest and then sequence the pull down DNA.
Take control of your ChIP-Seq data
Basepair is able to scale from a single sample to thousands with the help of parallel processing. No coding required.
Choose from ready-to-use ChIP-Seq analysis pipelines, hit Run, and get publication-ready results in hours, not days or weeks.
Basepair is used by top universities and peer-reviewer publications – with dozens of published studies using our software.
Publication-ready reports for your ChIP-Seq data
Our ChIP-Seq workflows include popular options such as alignment, QC, read counts, motif analysis, and peak calling, including a table of peaks and heatmaps. Below, you’ll find an overview of figures and interactive plots immediately available in your ChIP-Seq analysis report.
Check the quality of your raw ChIP-Seq data with our before- and after-trim QC charts. The QC score helps uncover any sequencing issues or contamination in your input data.
Read Count Flow
The read count flow helps provide a big-picture overview of usable reads at the end of the trimming, alignment, and deduplication processes. Think of the figure as a data analysis assembly line: input raw data, get an output of usable reads.
Peak Calling and Motif Analysis
Basepair can help you easily identify signal peaks in your data. Each peak is annotated as promoter, intronic, or intergenic, with the corresponing gene displayed. For any peaks found, we do a motif analysis to find overrepresented transcription factor binding sites.
Gene Body and Transcription Start Site
The gene body and transcription start site figures and corresponding heatmaps help you locate the appropriate signals. This ensures your sample has the expected pattern for the selected ChIP. If the signal-to-noise ratio is low, these figures can help you quickly identify any issues.
Basepair’s built-in Genome Browser allows for a birds-eye view of you raw genomic data. Our platform handles any conversions under the hood, presenting you with a visual overview of your ChIP-Seq data without additional file conversions or software necessary.
Set up your ChIP-Seq analysis in minutes
Try our free 14-day trial now. Explore our sample reports or upload your own ChIP-Seq data. Choose from a number of popular pipelines, such as QC, alignment, peak calling, motif analysis, and more. See why the world’s top institutions are using Basepair to save thousands of hours (and dollars) for their NGS data analysis needs.
Our reports have been published in dozens of peer-reviewed journals
Feedback from our customers
Support answers come fast and are always precise!Maria Hollnagel