by Charles Murphy | Oct 26, 2019 | Bioinformatics
Trimming for adaptors and low quality bases is important part of the analysis pipeline for sequencing data. Typically, after you isolate and fragment your RNA sample, adaptors are attached to the ends of the sequences that are needed for sequencing (see [1,2] for more...
by Amit | Mar 22, 2019 | Bioinformatics
NGS Data Analysis Bottlenecks One of the biggest bottlenecks in next generation sequencing (NGS) today is data analysis, which is surprising considering that we have massive compute available at our fingertips, the available infrastructure to process thousands of...
by Charles Murphy | Mar 5, 2019 | Bioinformatics
Background One of the most popular tools for pathway enrichment analysis for RNA-seq and microarray data is the Gene Set Enrichment Analysis (GSEA) tool by the Broad Institute [1,2]. The database of pathways most often used with GSEA is MSigDB, also by the Broad...
by Stuart Davis | Aug 27, 2016 | Bioinformatics
The performance of NGS alignment tools has continuously improved, but sorting the aligned data now takes more time than alignment. A big leap in sorting was recently initiated with SAMtools version 1.x, with support for parallel processing for sorting. We compared the...
